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Diagnosing stomach disease in pet reptiles

A popular “get well” card shows a racoon saying to a snake, “You wouldn’t get these stomach aches if you chewed your food properly.” Vets know, however, that indigestion in snakes and other reptiles often results not from swallowing food whole but from a parasitic infection. The gastrointestinal disease cryptosporidiosis represents a particularly severe problem: although it is rarely otherwise serious in mammals, reptiles seem especially prone to it and the condition is often fatal. Furthermore it is highly contagious, so early diagnosis would represent a good way to limit its spread among reptiles. Unfortunately, though, diagnosis is extremely difficult. Scientists at the University of Veterinary Medicine, Vienna have developed a test for the identification of the cryptosporidia that cause the condition, enabling them to assess its prevalence in pet lizards and snakes. The results are published in the current issue of the Journal of Veterinary Diagnostic Investigation.

Although known for over a century, cryptosporidiosis was believed to be an extremely rare condition and it only gained attention with the discovery that it can affect humans, especially immune-compromised individuals. It is caused by a single-cell parasite, one of a family known as cryptosporidia. Some cryptosporidia also infect reptiles, where after a sometimes lengthy incubation period they cause gastrointestinal problems even in otherwise healthy individuals. The condition is usually persistent and is presently impossible to cure. It is therefore important to minimize infections and in this regard reliable diagnostic procedures are essential. Diagnosis is based on the detection of parasites in faeces but is complicated by the fact that snakes in particular excrete parasites that they swallow together with their prey, so the presence of cryptosporidia in faeces does not necessarily mean the animals are infected. For this reason it is essential to be able to distinguish between “prey” cryptosporidia and those that cause infection in the snake. Barbara Richter and colleagues at the Institute of Pathology and Forensic Veterinary Medicine in the University of Veterinary Medicine, Vienna now report a DNA-based procedure able to determine not only whether cryptosporidia are present but also whether they are of mammalian or snake origin.

By means of the test, Richter was able to show that a particular type of cryptosporidium is present in about one in six samples from the popularly kept corn snake and in about one in twelve samples from the attractive leopard gecko, a lizard frequently found in reptile collections. These prevalence figures are far higher than previously suspected, showing the widespread nature of the disease. The corn snake in particular seems highly susceptible to infection. Worryingly, the new tool revealed that a large proportion of captive leopard geckos contain cryptosporidia of one form or another. It is possible that some of the infections do not inconvenience the host geckos but the animals nevertheless represent a source of infection for other reptiles that come into contact with them. Many reptile collections house a number of species together and there is therefore a significant risk of cross-species infection. The new diagnostic procedure represents a precise method for the early diagnosis of cryptosporidiosis in lizards and snakes, before the animals show symptoms of disease. Nevertheless, Richter still raises a cautionary note. “A further problem is that cryptosporidia are often present in faeces in very low numbers so it is easy to miss them in a single test. We are working to make our method more sensitive but it is very important to test the reptiles repeatedly. A negative result does not necessarily mean that the animal is really free of the parasite.”

Science Daily
June 15, 2011

Original web page at Science Daily

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Bizarre reptile challenges notion of crocodiles as ‘living fossils’

We all know that crocodiles are reptiles with long snouts, conical teeth, strong jaws and long tails. But according to researchers at Stony Brook University in New York, we don’t know what we thought we knew. Rather, some crocodiles possessed a dazzling array of adaptations that resulted in unique and sometimes bizarre anatomy, including blunt, pug-nosed snouts, pudgy bodies and short tails. These anatomical adaptations of the incredibly diverse group of reptiles called notosuchian crocodyliforms are brilliantly illuminated in a new Memoir of the Society of Vertebrate Paleontology. This massive, richly illustrated volume, edited by Drs. David W. Krause and Nathan J. Kley of Stony Brook, clearly dispels the notion that crocodiles are static, unchanging “living fossils.” The volume, which gives an account of fossil crocodyliform anatomy, is set for publication on December 8, 2010.

The epitome of crocodyliform anomaly is represented by Simosuchus clarki, which lived in Madagascar at the end of the “Age of Dinosaurs” (about 66 million years ago). First described preliminarily in 2000 from a well-preserved skull and partial skeleton, Simosuchus shattered the crocodyliform mold with its blunt snout, leaf-shaped teeth, and short, tank-like body covered in a suit of bony armor. “ Simosuchus is easily the most bizarre crocodyliform ever found,” declared Dr. Christopher Brochu, a leading expert on fossil crocodiles from the University of Iowa. Over the next decade, expeditions to Madagascar recovered more skulls and skeletons, now representing nearly every bone of Simosuchus. A reconstruction of this uncommonly complete fossil reptile and an interpretation of its place in the crocodile evolutionary tree became the subject of the new volume. “The completeness and preservation of the specimens demanded detailed treatment,” said Krause, Distinguished Service Professor in the Department of Anatomical Sciences at Stony Brook University. “It just seemed unconscionable to not document such fantastic fossil material of this unique animal.”

Brochu, who did not participate in the research, said that “very few crocodilians – even those alive today – have been subjected to this level of analysis. This reference sets a new standard for analyses of extinct crocodyliforms and is going to used for decades.” A separate chapter of the monograph is devoted to each of the major parts of the animal – skull, backbone, limbs, and armor. “The skull and lower jaw in particular are preserved almost completely,” said Kley, assistant professor in the Department of Anatomical Sciences at Stony Brook University. “This, combined with high-resolution CT scans of the most exquisitely preserved specimen, has allowed us to describe the structure of the head skeleton – both externally and internally – in exceptional detail, including even the pathways of the tiniest nerves and blood vessels.” But while it is easy to lose one-self in the details of these incredible fossils, one of the most amazing features is the overall shape of the animal. Two feet long, pudgy, with a blunt snout and the shortest tail of any known crocodyliform, Simosuchus was not equipped to snatch unsuspecting animal prey from the water’s edge as many modern crocodiles do.

“Simosuchus lived on land, and its crouched posture and wide body probably meant it was not very agile or fast,” said Joseph Sertich, a Ph.D. student in the Department of Anatomical Sciences at Stony Brook who participated in the research. In addition, its short, under-slung jaw and weak, leaf-shaped teeth show that it probably munched on a diet of plants. While the idea of a gentle, vegetarian crocodile is unusual to us today, the new memoir makes it easy to imagine Simosuchus ambling through its semi-arid grassland habitat, pausing to nip at plants and crouching low to hide from predators like the meat-eating dinosaur Majungasaurus. The paleontologists also found evidence that pointed to the evolutionary origin of Simosuchus. “Interestingly, an analysis of evolutionary relationships suggests Simosuchus’ closest relative lived much earlier, in Egypt,” said Sertich.

Science Daily
December 21, 2010

Original web page at Science Daily

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Grey wolves regain protected status

Efforts over 15 years to protect the species in the US have helped boost the wolves’ numbers. This has been so successful that last year the US Fish and Wildlife Service removed Endangered Species Act (ESA) protection for wolves in Idaho and Montana, though not in Wyoming. But last week, district judge Donald Malloy overturned that decision. He ruled that the law did not allow one part of the population to be hunted and another part protected. “This decision is a significant victory for wolves, for the integrity of the ESA, and for all Americans who care deeply about conservation,” says Rodger Schlickeisen, president of Defenders of Wildlife, which was a plaintiff in the case. However, Carolyn Sime, statewide wolf coordinator at Montana Fish, Wildlife and Parks, says the decision will be costly for farmers. “A minimum of 100 cattle and in some years up to 200 sheep are confirmed killed by wolves each year in Montana. That represents a significant financial burden if you’re a livestock owner,” Sime says. “The ability to manage a wolf population under fair chase hunting is now lost.”

There are more than 1700 grey wolves in the northern Rockies – an area that includes all of Idaho, Montana and Wyoming, plus portions of Washington, Oregon and Utah. Once abundant throughout North America, by the 1930s grey wolves had been exterminated in Montana, Idaho, Wyoming and south-western Canada. They were listed as endangered in 1974 under the ESA and reintroduced to the northern Rockies in the mid-1990s. “The service’s decision to delist the wolf in Idaho and Montana reflected the strong commitments from the states of Idaho and Montana to manage grey wolves in a sustainable manner,” says Tom Strickland of the US Fish and Wildlife Service. “Today’s ruling makes it clear this wolf population cannot be delisted until the state of Wyoming has instituted an adequate management programme, similar to those of Idaho and Montana.” “The million-dollar question is how to manage the wolves with these new legal hurdles,” says Sime. She says the parks service is looking at a range of options, including appealing the decision.

New Scientist
August 31, 2010

Original web page at New Scientist

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First genetic link between reptile and human heart evolution found

Scientists at the Gladstone Institute of Cardiovascular Disease (GICD) have traced the evolution of the four-chambered human heart to a common genetic factor linked to the development of hearts in turtles and other reptiles. The research, published in the September 3 issue of the journal Nature, shows how a specific protein that turns on genes is involved in heart formation in turtles, lizards and humans. “This is the first genetic link to the evolution of two, rather than one, pumping chamber in the heart, which is a key event in the evolution of becoming warm-blooded,” said Gladstone investigator Benoit Bruneau, PhD, who led the study. “The gene involved, Tbx5, is also implicated in human congenital heart disease, so our results also bring insight into human disease.” From an evolutionary standpoint, the reptiles occupy a critical point in heart evolution. While bird and mammalian hearts have four chambers, frogs and other amphibians have three. “How did hearts evolve from three to four chambers?” Bruneau said. “The different reptiles offer a sort of continuum from three to four chambers. By examining them, we learned a lot about how the human heart chambers normally form.”

He explained that with four chambers—two atria and two ventricles—humans and all other mammals have completely separate blood flows to the lungs and to the rest of the body, which is essential for us to be warm-blooded. When it comes to reptiles, such as turtles and lizards, there is debate about whether they have one or two ventricles, which are the pumping chambers. “The main question for us to understand the evolution of the heart was to identify the true nature of these early reptile ventricles and to figure out what controls the separation of the heart into left and right sides,” said Dr. Bruneau. To better understand reptile heart evolution, Dr. Bruneau’s team used modern molecular genetics to examine Tbx5. Mutations in the human gene that encodes Tbx5 result in congenital heart disease and, in particular, defects in the ventricular septum, the muscular wall that separates the ventricle into two sections. Tbx5 is a transcription factor, a protein that turns other genes on or off. In humans and other mammals, Tbx5 levels are high in the left ventricle and low in the right. The boundary of high and low levels is right where the septum forms to divide the ventricle into two parts. “Based on these observations,” said Dr. Bruneau, “we thought Tbx5 was a good candidate as a key player in the evolution of septation.”

The team looked at Tbx5 distribution in the turtle and the green anole lizard. During the early stages of heart formation in both reptiles, Tbx5 activity is found throughout the embryonic ventricular chamber. In the lizard, which forms only one ventricle, this pattern stays the same as the heart develops. However, in the turtle, which has a primitive septum that partially separates the ventricles into left and right sides, distribution of Tbx5 is later gradually restricted to the area of the left ventricle, resulting in a left-right gradient of Tbx5 activity. This meant that the gradient of Tbx5 forms later and less sharply in the turtle than in species with a clear septum, such as mammals, providing a tantalizing clue about how septation evolved. They then wanted to determine whether Tbx5 was really a main regulator of septation or merely a bystander. Mice were genetically engineered to express Tbx5 at a moderate level throughout the developing heart, just like in turtle hearts. By mimicking the turtle pattern, mouse hearts now resembled turtle hearts. The offspring from these mice died young and had only a single ventricle. This striking result conclusively showed that a sharp line delineating an area of high level of Tbx5 is critical to induce formation of a septum between the two ventricles. “This really nailed the importance of Tbx5 in patterning the heart to allow septation to occur,” said Dr. Bruneau.

During evolution, new genetic regulatory elements evolved to tell the Tbx5 gene to form a sharp boundary of Tbx5 expression. This resulted in two ventricles. Researchers will now work to identify those genetic regulatory mechanisms during the evolution of reptiles. The work also has important implications for the understanding of congenital heart defects, which are the most common human birth defect, occurring in one out of every one hundred births worldwide. Humans born with only one pumping chamber, resembling frog hearts, suffer the highest mortality and require extensive surgery as newborns. “Our study provides exciting new insights into the evolution of the heart, which had not been examined in over 100 years,” Dr. Bruneau explained. “In a larger context, it provides good support for the concept that changes in the expression levels of various regulatory molecules are important in evolution. From these studies we also hope to understand further how defects in septation occur in humans with congenital heart disease.”

Science Daily
September 22, 2009

Original web page at Science Daily

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Animal mating choices more complex than once thought

When female tiger salamanders choose a mate, it turns out that size does matter – tail size that is – and that’s not the only factor they weigh. Findings of a Purdue University study show that animals make more complex decisions about choosing mates than once thought. The results of Andrew DeWoody’s study, released Monday (June 8) in the journal Molecular Ecology, refute a theory that animals use major histocompatibility complex (MHC) genes as the sole basis for mate choice. Immunologists have long known that MHC genes play key roles in the immune response, but more recently behavioral ecologists have postulated that animal mate choice is often based on MHC-type because of the function of those genes. “Our data indicate that mate-choice decisions aren’t solely dependent on MHC, tail length, body size or any other single factor,” said DeWoody, a professor of genetics. “Mate choice is a complex process that takes many factors into account.”

DeWoody and David Bos, a former postdoctoral assistant who is now a continuing lecturer at Purdue, set out to see how much MHC genes affected mate choice in wild animals. Most prior research showed that an animal would choose a mate with MHC that is the most divergent from its own so that offspring will have more effective immune systems. Earlier studies in mice suggest that MHC diversity was the sole genetic basis for mate choice. But DeWoody said those studies used mice that were genetically the same in every way except for MHC. “Sure, mice might base mate-choice decisions wholly on MHC if there is no other consideration, if they don’t have any other factors to choose from,” DeWoody said. “But wild animals have a lot of different characteristics they can choose from, not just MHC.” Proteins encoded by MHC serve as the immune system’s sentry. MHC proteins expressed on the cell surface bind and display small peptides (bits of protein) to T-cells. T-cells interrogate the peptides and determine if they are foreign. If so, the immune response is activated. The more MHC diversity a person or animal has, the more peptides it is able to bind and display over to T-cells, making it less susceptible to infection.

DeWoody and Bos used tiger salamanders because of their unique mating habits in which females make the sole decision on choosing a mate. Males deposit spermatophores, or sperm packets, for females, who choose the ones that will be used to fertilize their eggs. The females are choosy because they want a mate whose attributes will increase the fitness of their offspring, DeWoody said. Using wild tiger salamanders, DeWoody and Bos gave each female a choice between two males. They checked the offsprings’ genotypes to identify parentage and found that the largest females chose the more MHC-similar mates, not the most divergent ones as expected under prevailing theory. The remaining females seemed to mate at random with regard to MHC. In addition to MHC, tail length plays a role in reproductive success. Male salamanders with longer tails were twice as likely as those with shorter tails to be chosen as sires. Bos said it’s possible that other factors outweighed MHC for some of the females. “There may very well be trade-offs,” Bos said. “Getting a mate with diverse MHC, large body size and other characteristics might be nice, but getting all of those characteristics might not be practical.”

Science Daily
June 30, 2009

Original web page at Science Daily

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Mosquito evolution spells trouble for Galapagos wildlife

The Galapagos giant tortoise and other iconic wildlife are facing a new threat from disease, as some of the islands’ mosquitoes develop a taste for reptile blood. Scientists from the University of Leeds, the Zoological Society of London (ZSL) and the Galapagos National Park have discovered that while its mainland ancestors prefer the blood of mammals and the occasional bird, the Galapagos form of the black salt marsh mosquito (Aedes taeniorhynchus) has shifted its behaviour to feed mainly on reptiles – primarily Galapagos giant tortoises and marine iguanas. The findings raise fears that these changes could devastate the islands’ unique native wildlife if a new mosquito-borne disease is introduced – a scenario which is increasingly likely with the continuing rise in tourism. Using genetic techniques, the researchers showed that the mosquito colonised the Galapagos around 200,000 years ago and was not introduced by humans as previously thought, giving them time to adapt to conditions in Galapagos. They have also found that unlike the mainland populations that normally live in mangroves and salt marshes along the coast, the Galapagos form of the mosquito can also breed up to 20 km inland and at altitudes of up to 700 metres.

The research team believe the shift in feeding behaviour is an adaptation to life in Galapagos, since the islands had few mammal species prior to the arrival of Man some 500 years ago. “When we started the work we thought that this species was also introduced by humans, so it was a surprise that it turned out to be so ancient,” says Arnaud Bataille, the University of Leeds and ZSL PhD student who carried out the work. “The genetic differences of the Galapagos mosquitoes from their mainland relatives are as large as those between different species, suggesting that the mosquito in Galapagos may be in the process of evolving into a new species.” Mosquitoes are known to transmit important wildlife diseases, such as avian malaria and West Nile fever. While there is no evidence that such diseases are currently present on Galapagos, the widespread presence of the mosquito, and the fact that it feeds on a broad range of the native species, means that any new disease that arrives from the continent could spread rapidly to a wide variety to wildlife throughout the islands. Due to its long isolation, Galapagos wildlife is not likely to have much immunity to new diseases, so the effects could be devastating.

“With tourism growing so rapidly the chance of a disease-carrying mosquito hitching a ride from the mainland on a plane is also increasing, since the number of flights grows in line with visitor numbers” says Dr Andrew Cunningham, from the Zoological Society of London, one of the authors of the study. “If a new disease arrives via this route, the fear is that Galapagos’ own mosquitoes would pick it up and spread it throughout the archipelago.” Rather than implementing control measures against Galapagos’ own unique mosquito, the research team argues that it is imperative that measures are taken to avoid introducing new diseases to the islands. The Ecuadorian government recently introduced a requirement for planes flying to Galapagos to have a residual insecticide treatment on the interior surfaces, and spraying in the hold and cabin on each flight. However, similar controls are yet to be implemented for ships.

Science Daily
June 19, 2009

Original web page at Science Daily

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Komodo dragons even more deadly than thought: Combined tooth-venom arsenal key to hunting strategy

A new study has shown that the effectiveness of the Komodo Dragon bite is a combination of highly specialized serrated teeth and venom. The authors also dismiss the widely accepted theory that prey die from septicemia caused by toxic bacteria living in the dragon’s mouth. Using sophisticated medical imaging techniques, an international team led by Dr Bryan Fry from the University of Melbourne have revealed that the Komodo Dragon (Varanus komodoensis) has the most complex venom glands yet described for any reptile, and that its close extinct relative Megalania was the largest venomous animal to have lived. The work will be published in the next issue of the journal Proceedings of the National Academy of Sciences.

“These large carnivorous reptiles are known to bite prey and release them, leaving the prey to bleed to death from the horrific wounds inflicted. We have now shown that it is the combined arsenal of the Komodo Dragon’s tooth and venom that account for their hunting prowess,” said Dr Fry from the Department of Biochemistry and Molecular Biology, Bio21 Institute at the University of Melbourne. “The combination of this specialized bite and venom seem to minimise the Dragon’s contact with its prey and this allows it to take large animals.” Komodo Dragons are native to the islands of Indonesia, with adult males weighing over 100kg, and exceeding 3 metres in length. They have around 60 highly serrated teeth which are frequently replaced during their lifetime. The researchers conducted a comprehensive study of the Komodo Dragon bite, employing computer techniques to analyze stress in a dragon’s jaws and compare them to those of a crocodile. The dragons were found to have much weaker bites than crocodiles, but magnetic resonance imaging (MRI) of a preserved dragon head revealed complex venom glands and specialised serrated teeth which create deep lacerations for entry of the venom.

“We believe that the dragon is able to weaken and immobilize their prey with a venomous bite that increases the damage done by their long serrated teeth,” said Dr Fry. The researchers located and surgically excised the glands from a terminally ill dragon at the Singapore Zoo, and used mass spectrometry to obtain a profile of the venom molecules. The team also analysed which toxin genes were expressed in the dragon’s venom gland. The effects of venom were also tested by the team and found to be similar to that of the gila monster and many snakes which cause a severe loss in blood pressure by widening blood vessels, thereby inducing shock in a victim. These findings may explain the observations by Dr Fry and others that Komodo Dragon prey become still and unusually quiet soon after being bitten. Bitten prey also bleed profusely, consistent with the team’s discovery that the venom was also rich in toxins that prolong bleeding. The researchers also examined fossils of the Dragon’s giant extinct relative Megalania (Varanus priscus). From similarities in skull anatomy they determined that this seven metre lizard would have used a similar venom and bite system, making it the largest venomous animal to have ever lived.

Science Daily
June 2, 2009

Original web page at Science Daily

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Lizards bask in the sun for a vitamin D boost

Keeping warm isn’t the only reason lizards and other cold-blooded critters bask in the sun. According to a study published in the May/June issue of Physiological and Biochemical Zoology, chameleons alter their sunbathing behavior based on their need for vitamin D. “It’s a longstanding assumption that thermoregulation is the only reason that lizards bask,” says Kristopher Karsten, a biologist at Texas Christian University who led the study. “Our results suggest that in addition to thermoregulation, vitamin D regulation appears to have a significant impact on basking behavior as well.” Chameleons, like humans and most other vertebrates, get vitamin D in two ways: They can absorb it from food, and they can produce it in their skin. In order to produce vitamin D, however, the skin must be exposed to UV radiation. To test whether chameleons alter their sunning behavior based on dietary vitamin D intake, Karsten observed the behavior of two different groups of chameleons. One group had high internal vitamin D levels, thanks to a diet of crickets dusted with a vitamin D powder. The other group ate regular crickets and had low vitamin D. The chameleons were then placed in individual outdoor enclosures that offered open area for direct sun, and a tree to offer filtered sun and shade.

Chameleons generally move from sun to shade throughout the day. But Karsten found that chameleons fed the low vitamin D diet compensated by increasing their exposure to the sun’s UV rays. Chameleons with high vitamin D diets, on the other hand, limited their UV exposure. “It appears that panther chameleons have the ability to gauge their internal vitamin D levels and alter their basking behavior accordingly,” Karsten says. “The chameleons were as effective as mathematically possible by our methods at regulating toward optimal UV exposure for their vitamin D profile,” he says. “We thought they were probably pretty good at regulating their UV exposure; we just didn’t think they’d be this good.” It’s not clear, however, by what mechanism they are able to sense their internal vitamin D levels, but Karsten thinks there may be a brain receptor sensitive to the vitamin. “Given the ability for panther chameleons to precisely, accurately and effectively adjust basking behavior as a direct result of vitamin D3, a brain vitamin D receptor, seems likely to occur in panther chameleons.”

Science Daily
May 4, 2009

Original web page at Science Daily

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Prehistoric turtle goes to hospital for CT scan in search for skull, eggs, embryos

Michael Knell carried a 75-million-year-old turtle into Bozeman Deaconess hospital recently, then laid it carefully on the bed that slides into the CT scanner. Hardly an ordinary patient, the turtle fossil was only the second in the world found with eggs inside it, said Knell, a Montana State University graduate student in earth sciences. His turtle (from the genus Adocus) came from the Grand Staircase-Escalante National Monument in Kanab, Utah. The previously described turtle, which was found in Alberta, is also an Adocus and lived about the same time. Knell wanted his fossil scanned to see if he could find a skull and more eggs and learn whether or not the eggs contained embryos. “It allows us to peer inside without digging into it,” Knell said. “It gives us a hands-off look without having to break anything.” “It’s very cool,” added Frankie Jackson, an MSU paleontologist who specializes in eggs. The CT machine looks like a giant doughnut with a narrow bed attached. Patients normally lie on the bed, then ride through the scanner while it takes images of a particular area. In this case, Knell wanted to scan the entire turtle. Although the edges of its shell were missing, the fossil measured about one foot across and weighed 40 to 50 pounds.

“It’s a lot easier than scanning people,” said CT technician Tanya Spence. Spence, who has scanned dinosaur fossils for MSU’s Museum of the Rockies, said she didn’t need to tell the fossil what it would experience during the CT scan. She didn’t need to ask the turtle to hold her breath. She didn’t worry about IV tubes or claustrophobia. “We get to see bodies everyday, but not these,” Spence said. “It’s fun.” Spence operated the scanner from an adjoining room while Knell and Jackson looked over her shoulders. As the CT scanner took cross-sections of the fossil, white images appeared — slice by slice — on Spence’s computer screen. “It’s very, very dense,” Spence said of the shell. “You can lose some detail when that happens. You have to penetrate enough to see, but you can sometime lose detail that way.” “Usually embryo bone doesn’t show up too well on a CT scan, so we might no be able to see embryos very well, but it could clue you in that you need to look at the eggs a little closer,” Jackson added.

As time went by, the scientists realized that the eggs weren’t showing up as clearly as they’d expected and they’d need to examine the images more closely. As a result, Spence saved her images to a disc and gave it to Knell. He and Jackson said they would contact the Museum of the Rockies, which has special software for looking at CT images from a variety of angles. The researchers expect to present their findings during the fourth international Symposium on Dinosaur Eggs and Babies. Knell’s adviser, David Varricchio, and Jackson are organizing the event, which will be held Aug. 8-10 at MSU. Knell said paleontologists have found about 10 different species of turtles and many fossilized egg shells in the hard sandstone of southern Utah. The team that found this Adocus in 2006 was led by Alan Titus, paleontologist at the Grand Staircase-Escalante National Monument, and Barry Albright, a researcher from the University of Northern Florida. Preparators learned that the turtle contained eggs after removing a section of its shell.

Adocus turtles generally laid small round eggs, about the same size as modern turtle eggs, Knell said. Based on what he knows about prehistoric turtles and living turtles, Knell said his Adocus was about a week away from laying the eggs when she died. He added that it’s rare to find turtle heads because the joint that connects them to the vertebrae is weak. Heads often get swept away and don’t stay with the bodies. Knell was hoping that his turtle’s skull had gone inside the shell before death.

Science Daily
May 4, 2009

Original web page at Science Daily

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Serious disease in pet lizards caused by new bacteria

Skin infections are common in pet lizards and can lead to fatal organ disease and septicaemia. Infections are particularly risky in lizards that are bred in captivity for release into the wild, as they can spread into the wild population. The cause of these diseases has been unclear but now researchers in Belgium have discovered a new bacterium responsible for dermatitis in desert lizards. According to research published in the September issue of the International Journal of Systematic and Evolutionary Microbiology, the discovery could help control the disease and protect endangered species. Desert-dwelling lizards belonging to the genera Agama and Uromastyx that live in the arid and desert areas of North Africa are now bred in captivity in Europe. “The establishment of healthy captive populations is an important tool for the conservation of threatened species,” said Professor An Martel from Ghent University, Belgium. “On the other hand, restocking of wild populations with captive bred animals carrying pathogens might compromise the survival of these wild populations. Skin diseases are highly prevalent in captive lizards.”

Dermatitis is the most important known bacterial disease of desert lizards that prevents successful captive populations from being established. One example is the captive breeding programme of the rare Oman dab lizard (Uromastyx thomasi) a joint project between Germany and Oman, to which pathogens like this may pose a real threat. “We isolated bacteria from five different desert lizards suffering from dermatitis, two agama lizards (Agama impalearis) and three spiny-tailed lizards (Uromastyx geyri and U. acanthinura),” said Professor Martel. “We could not identify the bacterium that was causing the disease, but the pathogen was the same in all five lizards.” The researchers looked at the genetic sequence of the bacterium and discovered it represents a new taxon and species. They have named the bacterium Devriesea agamarum (Devriesea referring to the veterinary microbiologist L.A. Devriese and agamarum after Agama, an Old World reptile). “We have demonstrated a causal relationship between this bacterium and skin lesions in desert-dwelling lizards,” said Professor Martel. “This microbe is also related to bacteria that cause skin infections in humans.”

The cases of dermatitis and septicaemia from which the new bacterium Devriesea agamarum was isolated are highly prevalent, especially in captive lizards. The researchers hope the identification of this species will contribute to our understanding of lizard skin disease and help develop control measures. “In the future we would like to study host-pathogen interactions, design treatments and investigate the use of a vaccination to prevent the development of disease caused by Devriesea agamarum,” said Professor Martel.

Science Daily
November 11, 2008

Original web page at Science Daily

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Alligators’ muscles move lungs around for sneaky maneuvers in water

Without a ripple in the water, alligators dive, surface or roll sideways, even though they lack flippers or fins. University of Utah biologists discovered gators maneuver silently by using their diaphragm, pelvic, abdominal and rib muscles to shift their lungs like internal floatation devices: toward the tail when they dive, toward the head when they surface and sideways when they roll. It allows them to navigate a watery environment without creating a lot of disturbance,” says doctoral student T.J. Uriona. “This is probably really important while they are trying to sneak up on an animal but don’t want to create ripples.” The discovery in American alligators suggests “special muscles that manipulate the position of the lungs — and thus the center of buoyancy — may be an underappreciated but important means for other aquatic animals to maneuver in water without actively swimming,” says C.G. Farmer, an assistant professor of biology. Those animals include crocodiles, African clawed frogs, some salamanders, turtles and manatees, she adds, noting that the use of muscles to move the lungs may be “incredibly important or you would not see it evolve repeatedly.”

The researchers found that alligators are somewhat like pilots using controls to adjust an aircraft’s pitch and roll, except the reptiles’ controls are muscles that help them shift their lungs backward to dive, forward to surface or sideways to roll. Farmer says the new study asked how gators “manage to maneuver so gracefully without the fins and flippers used by fish, seals and other adept swimmers.” “The secret to their aquatic agility lies in the use of several muscles, such as the diaphragmatic muscle, to shift the position of their lungs. The gases in the lungs buoy up the animal, but if shifted forward and backward cause the animal to pivot in a seesaw motion. When the animals displace gases to the right or left side of the body, they roll.” Uriona says that during the Triassic Period, which began 250 million years ago, the crocodilian ancestors of alligators were cat-sized animals that lived only on land. “Until now, it was believed the diaphragmatic muscle evolved to help them breathe and run at the same time,” he says. “Showing they are actually using it to move around in water gives an alternative explanation for why the muscle evolved.” It also suggests the muscle didn’t evolve until after crocodilians took to the water during the Cretaceous Period, which began 145 million years ago. During that time alligators’ ancestors also evolved a flattened skull, shorter limbs and a big tail.

Science Daily
April 1, 2008

Original web page at Science Daily

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How to give a gator heartburn

The way to an alligator’s stomach is through its heart–via the left aorta, to be precise. That’s the upshot of a new study that may have finally explained the enigmatic crocodilian circulatory system. Crocs are able to send deoxygenated blood directly to their stomach, and researchers show that strategy comes in handy when the reptiles need to quickly digest enormous helpings of meat and bone. Like birds and mammals, crocodilians–the group that includes alligators, caimans, and crocodiles–have a four-chambered heart. They can completely divide their blood flow in the same way we do: Deoxygenated blood returns to the heart from the body, heads straight to the lungs to pick up oxygen, returns to the heart, and then gets pumped to the rest of the body. Although the four-chambered system is considered more efficient, crocs still sometimes engage an alternative primitive flow pattern characteristic of other reptiles. They can bypass the pulmonary artery that carries blood to the lungs, sending spent blood straight back to the digestive system through the left aorta. Since the early 1800s, scientists have envisioned many functional explanations for this shunting architecture–from keeping CO2 out of the lungs during dives to speeding digestion–but experimental data have remained scarce.

To test the digestion hypothesis, zoologist C. G. Farmer of the University of Utah in Salt Lake City and colleagues performed a series of experiments on young American alligators. First, they implanted probes around the left aortas of five gators and found that the animals shunted more deoxygenated blood to their stomachs after eating. Then, the researchers operated on a group of nine gators, closing off the left aorta. The surgery prevented shunting and forced the animals’ circulation to run in the fully divided mammalian pattern. (The gators appeared healthy despite the change.) A control group underwent only sham surgeries.

After they recovered, the 2-kilogram alligators feasted on chopped steak, and the researchers measured their gastric acid secretion. As the team reports in the March/April issue of Physiological and Biochemical Zoology, the animals with intact shunting abilities produced more stomach acid than did the altered reptiles, especially when it was warm. In fact, they produced 10 times more than the greatest amount recorded in any animal, apparently using the CO2-rich blood to supply stomach glands that use CO2 to make gastric acid. The “acid secretion seemed ludicrous it was so high,” Farmer says. All that stomach acid should be good for digesting large, bony meals. Indeed, x-rays showed that shunting gators with more acidic tummies finished digesting a bony ox tail days ahead of their nonshunting counterparts. Because alligators rely on external heat to warm their bodies, they can’t always maintain the ideal temperature for digestion–so processing huge meals as quickly as possible is “like making hay while the sun shines,” Farmer says.

Gordon Grigg, a zoologist at the University of Queensland in Brisbane, Australia, calls the study “fantastic” and says he is “in awe” of the technical challenges the researchers overcame. But he won’t be completely convinced, he says, until researchers can show that blocking the left aorta doesn’t decrease overall blood supply to the stomach, which might also slow digestion.

ScienceNow
February 19, 2008

Original web page at ScienceNow

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Genes versus heat – a reptile sex trigger

High temperatures can make an Australian lizard that is genetically male develop into a female. The finding throws new light on how sex is determined in reptiles. For most reptiles, a gene on a sex chromosome triggers an embryo to develop as either a male or a female. In some species, males have an X and a Y chromosome, while females are XX, as in mammals. In other species such as birds, males are ZZ, while females are ZW. But for a third group, which includes all crocodiles, alligators and marine turtles, temperature, rather than a gene on a sex chromosome, triggers either male or female differentiation. Extreme low or high temperatures generally lead to more females.

Now, a team led by Alex Quinn at Canberra University in Australia, has found that the central bearded dragon (Pogona vitticeps) is susceptible to both types of sex trigger, and that temperature can override its genetic gender. When the team incubated eggs at relatively high temperatures – between 34°C and 37°C – the majority of embryos that had ZZ sex chromosomes (genetically male), hatched as females. The team thinks the bearded dragon represents a transitional form, in evolutionary terms, between the two main methods of sexual determination. The research shows that, for the bearded dragon at least, the W chromosome is not necessary in producing a female. Furthermore, the team discovered that a double dose of a particular gene on the Z chromosome is crucial for maleness, and that this gene is inactivated by high temperatures.

“The possibility that there is a male-determining, dosage-dependent gene on the Z chromosome of bearded dragons is an important insight,” says Quinn, “because to date, scientists have discovered the master sex-determining gene only in mammals and a single species of fish.” The team plans to hunt for that master gene in the bearded dragon. They also want to investigate how widespread the phenomenon of temperature sex reversal really is in reptiles. If many other reptiles with sex chromosomes are also susceptible to temperature, this would broaden the number of species that could be vulnerable to climate change. “The concern is that the current rate of climate warming could be too rapid for these species to adapt to, and this could potentially result in heavily skewed sex ratios, and even population crashes in some cases,” Quinn says.
Source: Science (vol 316, p 411)

New Scientist
May 01, 2007

Original web page at New Scientist

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Reptiles join amphibians in mysterious decline

The catastrophic declines in frog and salamander populations in recent years may be even more widespread than had been feared – and the devastation seems to extend to reptiles. Steven Whitfield at Florida International University in Miami, US, and colleagues studied data on ground-dwelling reptiles and amphibians collected over the past 35 years at La Selva Biological Station in Costa Rica. The counts show a decline of around 75% in reptiles and amphibians in native forest since 1970. But the numbers of both increased in abandoned cacao plantations, by 4% annually for amphibians, and 2.7% per year for reptiles. Fungal diseases or pesticide contamination, blamed for amphibians’ decline elsewhere, are unlikely to be behind the declines at La Selva, since they would affect abandoned plantations as well as native forest. The researchers suggest the cause may be a change to a warmer, wetter climate, which reduces tree growth and may lead to a thinner layer of leaf litter, where the animals live. Cacao plantations (which produce cocoa) produce a heavier layer of this leaf litter. Pesticides and fungi have previously been blamed for the decline in Costa Rica’s mountain amphibians.
Source: Journal reference: Proceedings of the National Academy of Sciences.

New Scientist
May 1, 2007

Original web page at New Scientist

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Dinosaurs breathed like birds

Dinosaurs’ hollow bones may have given them the puff to lead active lifestyles. A fossil find shows that the group of dinosaurs that included Velociraptor and Tyrannosaurus rex probably used the same super-efficient respiratory system that birds have today. The fossil, which is of a carnivorous dinosaur called Majungatholus atopus, shows that its bones included spaces for storing air. This would have allowed the species to have the quick metabolism necessary for an active predatory lifestyle.

Birds have fast metabolic rates thanks to their efficient way of extracting oxygen from the air. They have two lungs, as mammals do, but the airflow through them is controlled by a complex system of air sacs throughout the body. Most birds have nine such sacs, which also extend through their hollow bones. The fossil indicates that these animals had the potential for a high metabolic rate. “This study paints a clearer picture of how these organisms would have existed in their environment,” says O’Connor. “It indicates that these animals had the potential for a high metabolic rate.”

Birds are thought to be direct descendants of theropod dinosaurs, the group to which M. atopus belongs.
Palaeontologists already have evidence that the extinct creatures were similar to their descendants, with high growth rates, bird-like sleeping postures and even feathers. O’Connor compared the structure of air sacs in M. atopus’s vertebrae to those in more than 200 living birds. The structures were very similar, they report in Nature.”This study forms part of an increasingly robust story that says birds are essentially dinosaurs, but smaller,” says Paul Barrett, a palaeontologist at the Natural History Museum in London. “Using functional work in live animals is a nice addition, and perhaps now you could go as far as saying dinosaurs had a bird-like metabolism.” The study shows that the efficient breathing system of birds is older than previously thought, but Barrett thinks there is more to come: “To me it seems that a breathing system like this is of more ancient origin, from nearer the base of the dinosaur family tree.” He says that finding older dinosaur fossils would support this, and perhaps show that other bird-like characteristics are older than suspected.

Some palaeontologists still dispute that dinosaurs were closely related to birds, and have suggested that their breathing systems were more like those of crocodiles. “This work is another nail in the coffin for that competing theory,” says Barrett.

Nature
August 2, 2005

Original web page at Nature

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Bird bones offer insights to dinosaur sexing

A type of bone that female birds use as a calcium reserve for making eggshell has been found inside the fossilised thigh bone of a Tyrannosaurus rex. This new forensic clue could be used to sort the bones of female predatory dinosaurs from those of the males. Discerning the sex of fossilised animals is notoriously difficult. The obvious differences are in soft tissue that is not preserved. Some sex-specific traits are preserved in bones, such as differences in size or skull adornment. But without having seen living animals it is hard to be certain which sex is which, and if those differences are between individuals, species or even between the sexes of a single species.

So far the only sure way of identifying a dinosaur’s sex has been to find unlaid eggs inside a female, and only a couple of such fossils have been discovered – a Sinosauropteryx and an oviraptor. Now a structure called medullary bone, previously found only inside the leg bones of living female birds, has been identified inside the same T. rex femur which seems to have preserved blood vessels for nearly 70 million years.

Densely mineralised and rich in blood vessels, medullary bone can be deposited quickly when females ovulate and can quickly release calcium when it is needed to form eggshell, says Mary Schweitzer at North Carolina State University in Raleigh, US, and one of the study team. The calcium reserves are critical for female birds because they have strong but lightly-structured bones. Without medullary bone deposited in their marrow cavities, the calcium used for egg shells would come from their bones, giving them avian osteoporosis. Forming the shells depletes medullary bone, which in living birds remains depleted during brooding and until their next ovulation.

Schweitzer recognised the distinctive structure of medullary bone under the microscope. In the study, she shows the T. rex medullary bone is similar to that found in living emus and ostriches – large animals which are close to the evolutionary roots of modern birds. The similarity bolsters the case that birds evolved from theropod dinosaurs – two-legged predators such as Velociraptor and T. rex.

Living crocodiles do not have medullary bone, Schweitzer notes, and there is no evidence as yet that non-theropod dinosaurs did. “We have never seen anything like this in another dinosaur,” adds Jack Horner of Montana State University in Bozeman, US, and also on the team. Ancestral theropods were small animals with hollow bones, while other dinosaurs had very small marrow cavities with no room for such structures.

Journal reference: Science (vol 308, p 1456)

New Scientist
June 21, 2005

Original web page at New Scientist

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Dinosaur eggs offer clues on reproduction

The rare discovery of eggs inside a dinosaur has given scientists new clues about the reproductive biology of the creatures and more support for the theory that birds came from dinosaurs. The pair of shelled eggs is the first of its kind found inside a dinosaur, said researchers who reported the discovery in Friday’s issue of the journal Science.

Scientists found the dinosaur produced eggs in some ways like a crocodile and in other ways like a bird. Crocodiles and similar primitive reptiles have two ovaries enabling them to lay a clutch of eggs. Birds have a single ovary and can only lay one egg at a time. The dinosaur’s egg-producing capability lay somewhere in between, suggesting a link with the modern bird, researchers said. It could produce more than one egg, but only one from each ovary at a time.

The theory that birds came from dinosaurs has been supported by many researchers, said Tamaki Sato of the Canadian Museum of Nature in Ottawa. But this latest research helps advance it, she added, calling it “strong evidence.” There have been previous findings of round objects around dinosaur skeletons and scientists have suspected they might be eggs but because they did not have shells, there wasn’t certainty, Sato said. “You have egg shells with this one,” she said of the specimen at the National Museum of Natural Science in Taiwan that was excavated from China. “This is the first time for sure.”

The scientists studied a dinosaur from a group of dinosaurs called oviraptorosaurians. This type of dinosaur — probably 10 feet to 13 feet long — is a subgroup of the theropods, thought to have been the ancestors to modern birds. The remains of the shelled eggs looked like pineapple-sized potatoes. The similar size of the eggs suggests the creature’s two oviducts each produced a single, shelled egg at the same time, the report said. Matt Carrano, curator of dinosaurs at the National Museum of Natural History in Washington, said the findings provide greater insights into the biology of the dinosaur and the evolution of birds. “It’s a window into a particular stage of evolution,” he said. “This particular dinosaur has characteristics that are birdlike but retains reptillian-like features.” “You have oviducts but they’re only doing one egg at time. Its biology is half way there between a bird and reptile.” Carrano also said it tells something about birds.

“The evolution of one egg at a time happened very early, before birds could fly, and then the evolution of only one oviduct happened later,” he said. “That might be related to the origin of flight,” he said, explaining that maybe birds wanted to lighten their body and so developed one oviduct.

Yahoo
April 26, 2005

Original web page at Yahoo

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Blood vessels recovered from Tyrannosaurus rex bone

Palaeontologists have extracted soft, flexible structures that appear to be blood vessels from the bone of a Tyrannosaurus rex that died 68 million years ago. They also have found small red microstructures that resemble red blood cells. The discovery suggests biological information can be recovered from a wider range of fossil material than realised, which would greatly help the tracing of evolutionary relationships. The preservation found by the researchers is extraordinary – far better than traditionally expected in dinosaur bone. But that may be because researchers have not been looking hard enough at their finds. Mary Schweitzer at North Carolina State University, US, has also extracted similar soft structures from a few other dinosaur bones.

The leg bone came from a skeleton called B-rex found in a remote canyon in South Dakota, in 2000 by a member of Jack Horner’s research team at the Museum of the Rockies in Montana. The 107-centimetre-long femur – small for a T. rex – was intact when found, and its hollow interior had not been filled with minerals. That is unusual for a long-buried bone. However, with a protective plaster jacket built around it, the bone was too heavy for a helicopter to retrieve it from the remote site and it had to be broken in half. When Horner’s group split the bone, they carefully took samples for Schweitzer, then working at the Museum of the Rockies.

Bones are built by cells called osteocytes which are nourished by a rich fabric of blood vessels. The osteocytes secrete proteins which collect the calcium compounds that give bones their strength. To see what remained of this internal structure, Schweitzer soaked samples of the core of the bone in a solution that dissolved the calcium compounds. This left what she describes as “a flexible vascular tissue that demonstrates great elasticity and resilience”. For comparison, she then examined ostrich bones, as these birds are the largest and closest living relatives of T. rex. She found similar structures when she removed the calcium from the ostrich bones and treated the mixture with enzymes to break down collagen fibre in the bony matrix.

Other researchers have previously recovered traces of protein from dinosaur bones, and indeed just two weeks ago Schweitzer reported traces of protein in 70 million year old dinosaur eggs. “[The T. rex paper] suggests that biological and biochemical information might be recoverable from a wide range of fossil material,” says Angela Milner of the Natural History Museum, in London, UK, who has detected proteins in Iguanadon bone. “There certainly seem to be blood vessels,” she told New Scientist.

The next step will be to isolate proteins and try to sequence them. Comparing protein sequences could help trace relationships with other prehistoric beasts and with animals alive today. Schweitzer decline to discuss DNA because she does not work with it, but DNA is far less stable than proteins so is usually broken into fragments, even in tissue that has been frozen since the ice age.

Journal reference: Science (vol 307, p 1952)

New Scientist
April 12, 2005

Original web page at New Scientist

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Effect of regulation and education on reptile-associated salmonellosis

Reptiles have become increasingly common as domestic pets, and with them reptile-associated Salmonella infections in humans. From 1990 to 2000, a total of 339 reptile-associated Salmonella cases were reported in Sweden. In 1996, as part of its efforts to adapt its import regulations to those of the European Union, Sweden no longer required certificates stating that imported animals were free of Salmonella. A subsequent increase was noted in the incidence of reptile-associated cases from 0.15/100,000 in the period 1990–1994 to 0.79/100,000 in 1996 and 1997. After a public education campaign was begun through the news media, the incidence dropped to 0.46/100,000. Children were the most affected age group among patients (incidence 1.3/100,000). Salmonella enterica serotype Enteritidis was the most frequent serotype (24% of isolates), followed by S. Typhimurium (9% of isolates). Import restrictions and public information campaigns are effective public health measures against reptile-associated salmonellosis.

Salmonellosis is an important worldwide health problem, affecting both humans and animals. In the United States, Salmonella causes an estimated 1.4 million episodes of infection and 400 deaths annually in humans. Salmonella usually causes a moderate gastrointestinal disorder, but it may result in more severe disease, such as bacteremia or meningitis, sometimes with fatal outcome. For decades, reptiles have been recognized as a source of human salmonellosis. Salmonella species were first isolated from snakes, turtles, and lizards in the 1940s, and more recent studies have shown that at least 50%–90% of these animals are carriers of Salmonella. The bacteria are excreted intermittently in the feces but can also be isolated from the cloacae, skin, and throat of water-living reptiles.

Reptiles have become increasingly common as domestic pets. In Canada, pet turtle–associated salmonellosis was recognized as a serious health problem in the 1960s and 1970s, and the country banned imported turtles in 1975.
Sweden has a long tradition of combating and controlling Salmonella in feed, animals, and humans, dating back to a large outbreak of salmonellosis in 1953 that affected >9,000 persons and caused 90 deaths. From 1970 to 1994, these control measures also included import restrictions on reptiles; anyone who wanted to import reptiles or turtles needed a certificate stating that the animals were free of Salmonella, and importing of turtles with shells <10 cm was not allowed. In 1995, Sweden dropped its requirement for a Salmonella certificate and instead required an import permit issued by the Swedish Board of Agriculture. When Sweden became a member of the European Union (EU) in January 1995, a number of new rules were adopted. As a result of these changes, Sweden no longer required import permits for reptiles and turtles, and it also lifted the import ban on small turtles. The adaptation of import regulations for reptiles took effect on March 1, 1996. Emerging Infectious Diseases
March 29, 2005

Original web page at Emerging Infectious Diseases

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Ancient crocodile found in Australia

A new species of crocodile which lived 40 million years ago has been discovered in tropical Australia, filling a gap in the evolution of the prehistoric-looking crocodile, researchers said on Thursday. Two nearly complete skulls and a lower jaw of a new species of crocodile that belonged to a group called Mekosuchinae were unearthed by miners in the northern state of Queensland, said Australia’s Monash University researcher Lucas Buchanan. “There is a big gap from about 30 to 60 million years ago of which we have no clue, except for these guys,” Buchanan told Reuters on Thursday.

Buchanan said the new species of crocodile was living in the early Tertiary period, from 65 million years ago until five million years ago, during which time climate change possibly had a major impact on the evolution of the modern-day crocodile. During the period, Australia and Antarctica broke apart and most of eastern Australia became warmer, leading to an increase in rainforests — an ideal environment for crocodiles. Buchanan said the new species of crocodile was very similar to the modern-day freshwater crocodile, suggesting the modern crocodile had changed little in millions of years of evolution.

“This croc would have looked much like a modern freshwater crocodile, which is the beautiful thing about crocodiles. They found something that works and stuck with it all through history,” he said. However, the ancient crocodile had sharper and laterally compressed teeth enabling it to sheer prey and an extra jaw muscle to give a stronger, more powerful bite. Buchanan said researchers were examining more crocodile fossils and hoped to add to the crocodile evolution puzzle. “It will also help us place this unique Australian group with the bigger picture of where they fit in with other lines of crocodiles,” he said.

Yahoo
March 15, 2005

Original web page at Yahoo